Protein–Protein Interactions: Surface Plasmon Resonance

Ligand and Analyte PreparationMaterial and Buffer PreparationPh ScoutingImmobilization of Ligand Using Amine CouplingImmobilization of Control LigandAnalyte Binding AnalysisRegeneration OptimizationAffinity MeasurementsAffinity Data Analysis1 Kinetics Data Analysis 1. The purified proteins must be dialyzed ON (overnight) at 4 °C against the running buffer to be used in the SPR experiment (see Notes 7 and 8 ).2. Check the purity and the stability of the proteins at least 1 day before doing the experiment. This can be done by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and coom... 1. The purified proteins must be dialyzed ON (overnight) at 4 °C against the running buffer to be used in the SPR experiment (see Notes 7 and 8 ).2. Check the purity and the stability of the proteins at least 1 day before doing the experiment. This can be done by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and coom...3. Thaw proteins on ice for 30 min.4. Centrifuge proteins at 16,000 × gfor 20 min at 4 °C.展开选择时，新内容将添加到焦点当前区域上方在link.springer上Badreddine Douzi标记:Publish Year:2020SPR